By Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov
"A mirrored image of the serious examine of the consequences of electromagnetic fields on residing tissues that has taken position over the past numerous many years, this booklet discusses the theoretical and experimental facts and concerns the results of sturdy electromagnetic fields and/or electrical pulses and their value in medication and biology. The authors current the fundamental recommendations utilized in electroporation and the complex equipment for production of nanopores, highlighting their uncomplicated technology and scientific purposes. issues contain nano electroporation, vintage electroporaiton, experimental proof for electroporation of dwelling cells, and electroporation for melanoma and wound healing"--Provided via writer. learn more... content material: fundamentals of Electroporation actual Chemical thought of Membrane Electroporation and Electrotransfer of Biogenic brokers, E. Neumann and S. Kakorin Bioelectric impact of severe Nanosecond Pulses, K.H. Schoenbach triggered Transmembrane Voltage-Theory, Modeling, and Experiments, T. Kotnik and G. Pucihar Electroporation: A evaluate of uncomplicated difficulties in conception and test, M.S. Markov Mechanisms of Electroporation in Lipid structures Electrodeformation, Electroporation, and Electrofusion of Cell-Sized Lipid Vesicles, R. Dimova Fluorescent equipment in evaluate of Nanopore Conductivity and Their Computational Validation, M. Kotulska, W. Dyrka, and P. Sadowski Electroporation of Lipid Membranes: Insights from Molecular Dynamics Simulations, M. Tarek and L. Delemotte Nanoscale Restructuring of Lipid Bilayers in Nanosecond electrical Fields, P.T. Vernier Mechanisms of Electroporation of Cells Nanopores: a different Transmembrane Passageway in Electroporated Cells, A.G. Pakhomov and O.N. Pakhomova version of telephone Membrane Electroporation and Transmembrane Molecular shipping, D. Miklavcic and L. Towhidi Kinetics of Pore Formation and Disappearance within the cellphone in the course of Electroporation, G. Saulis the heart beat Intensity-Duration Dependency for mobile Membrane Electroporation, D. Miklavcic, G. Pucihar, A.M. Lebar, J. Krmelj, and L. Towhidi Mechanisms of Electroporation in Tissues Drug-Free, strong Tumor Ablation via Electroporating Pulses: Mechanisms That Couple to Necrotic and Apoptotic mobilephone loss of life Pathways, A.T. Esser, K.C. Smith, T.R. Gowrishankar, and J.C. Weaver Gene Electrotransfer: From easy methods to Preclinical functions, J.-M. Escoffre, A. Paganin-Gioanni, E. Bellard, M. Golzio, M.-P. Rols, and J. Teissie Technical concerns Modeling electrical box Distribution In Vivo, N. Pavselj, A. Zupanic, and D. Miklavcic thoughts of Electroporation Pulse iteration and evaluation of electrical Pulse turbines for cellphone and Tissue Electroporation, M. Rebersek and D. Miklavcic iteration of Ultrashort Pulses, J.F. Kolb Nanosecond Pulsed electrical box supply to organic Samples: problems and strength strategies, A. Silve, J. Villemejane, V. Joubert, A. Ivorra, and L.M. Mir purposes of Electroporation Translation of Electroporation-Mediated DNA supply to the medical institution, L.C. Heller and R. Heller medical Electrochemotherapy: The Italian adventure, C.R. Rossi and L.G. Campana Tumor Blood Flow-Modifying results of Electroporation and Electrochemotherapy-Experimental proof and Implications for the remedy, T. Jarm, M. Cemazar, and G. Sersa lectrochemotherapy as a part of an Immunotherapy technique within the therapy of melanoma, J. Gehl ombined electric box and Ultrasound: A Nondrug-Based technique for Tumor Ablation, P.F. Forde, C. Twomey, G.C. O' Sullivan, and D. M. Soden mixed Modality treatment: Electrochemotherapy with Tumor Irradiation, G. Sersa, S. Kranjc, and M. Cemazar Irreversible Electroporation in drugs, B. Rubinsky nutrients and Biomaterials Processing Assisted by means of Electroporation, N. Lebovka and E. Vorobiev In Vivo Electroporation: an immense harm Mechanism in electric surprise Trauma, I. Barakat, J. Gallaher, H. Chen, and R.C. Lee Index summary: Reflecting the serious learn of the results of electromagnetic fields on dwelling tissues that has taken position through the years, this name summarizes the experimental findings and theories regarding permeabilization of biomembranes through pulsed electrical fields. it truly is meant commonly for biomedical and actual scientists, engineers, and clinicians. learn more...
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Extra info for Advanced electroporation techniques in biology and medicine
15 Influence of 10 ns electrical pulses with electric field amplitudes of 125 kV/cm on the aggregation of platelets. The results obtained with various pulse numbers are compared to the effect of thrombin. Platelet aggregation was measured in a Chrono-Log Whole Blood Aggregometer (model 560-VS). The amount of transmitted light that passed through the sample cuvette (containing washed human platelets) was compared to the reference cuvette that contained buffer only. As platelets aggregated, there was an increased amount of light transmitted through the sample cuvette, which reflected increased platelet aggregation.
Dielect. Electr. , 14, 1088, 2007. , thrombin) in many nonexcitable cells (Taylor, 2006). , the two processes are coupled. , 2008). , 2008). , 2001). , PDGF and transforming growth factor beta [TGF-β]). The release of this growth factor is essential for wound healing. In addition to growth factor release, recent studies have also shown that nsPEFs have an antibacterial effect. The concentration of Staphylococcus aureus decreased considerably after treatment with nsPEF-activated platelet gel and decreased significantly compared with bacteria treated with thrombin.
Mol. , 53, 55–63. , 2009, Design of a special dielectric lens for concentrating a subnanosecond electromagnetic pulse on a biological target (Special issue on Bioelectrics), IEEE Trans. Dielect. Electr. , 16, 1364–1375. , 42, RS6S27. , 2002, Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: Apoptosis induction and tumor growth inhibition, IEEE Trans. , 30, 286–292. , 17, 1493. , 22, 785–796. , 2004, Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms, Physiol.
Advanced electroporation techniques in biology and medicine by Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov