Download e-book for iPad: Antisense Technology, Part B by John N. Abelson, Melvin I. Simon, M. Ian Phillips

By John N. Abelson, Melvin I. Simon, M. Ian Phillips

ISBN-10: 0080496717

ISBN-13: 9780080496719

ISBN-10: 012182215X

ISBN-13: 9780121822156

Antisense expertise is the power to control gene expression inside of mammalian cells offering robust experimental techniques for the research of gene functionality and gene law. for instance, equipment which inhibit gene expression allow stories probing the traditional functionality of a selected product inside a mobilephone. Such technique can be utilized in lots of disciplines equivalent to pharmacology, oncology, genetics, mobile biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity might be a really very important instrument in biomedically-oriented research.The significantly acclaimed laboratory average for greater than 40 years, equipment in Enzymology is likely one of the such a lot hugely revered courses within the box of biochemistry. because 1955, each one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless correct today-truly a vital ebook for researchers in all fields of existence sciences.

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ABELSON METHODS IN ENZYMOLOGY VOLUME 319. Singlet Oxygen, UV-A, and Ozone (in preparation) Edited by LESTER PACKER AND HELMUT SIES VOLUME 320. Cumulative Subject Index Volumes 290–319 (in preparation) VOLUME 321. Numerical Computer Methods (Part C) (in preparation) Edited by MICHAEL L. JOHNSON AND LUDWIG BRAND VOLUME 322. Apoptosis (in preparation) Edited by JOHN C. REED xxxv Section I Antisense Receptor Targets This . Page Intentionally Left Blank [1] ANTISENSE AND OPIOID RECEPTOR SIGNALING IN CNS 3 [1] In Vivo Modulation of G Proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides By JAVIER GARZO´ N, ISABEL DE ANTONIO, and PILAR SA´NCHEZ-BLA´ ZQUEZ Introduction Opioids promote their effects by acting on cellular receptors located in the cell membrane.

Sci. A. 88, 7595 (1991). A. Szklarczyk and L. Kaczmarek, J. Neurosci. Methods 60, 181 (1995). 15 Y. Yaida and T. S. , Regul. Pept. 59, 193 (1995). 16 B. J. Chiasson, J. N. Armstrong, M. L. Hooper, P. R. Murphy, and H. A. Robertson, Cell. Mol. Neurobiol. 14, 507 (1994). 17 B. D. Shivers, I. Killisch, R. Sprengel, H. Sontheimer, M. Ko¨hler, P. R. Schofield, and P. H. Seeburg, Neuron 3, 327 (1989). 14 [2] TARGETED GABAA RECEPTORS 27 mRNA is often used in in vivo antisense studies involving CNS proteins and has been successful in a number of studies targeting different neuronal proteins.

269, 13880 (1994). 13 S. Offermanns, T. Wieland, D. Homann, J. Sandmann, E. Bombien, K. Spicher, G. Schultz, and K. H. Jakobs, Mol. Pharmacol. 45, 890 (1994). 14 C. W. Taylor, Biochem. J. 272, 1 (1990). 15 A. D. Strosberg, Eur. J. Biochem. 196, 1 (1991). 16 J. Garzo´n, M. A. Castro, J. L. Juarros, and P. -Pharmacol. Lett. 54, PL191 (1994). 17 J. Garzo´n, A. Garcı´a-Espan˜a, and P. Sa´nchez-Bla´zquez, J. Pharmacol. Exp. Ther. 281, 549 (1997). 18 J. Garzo´n, M. Castro, and P. Sa´nchez-Bla´zquez, Eur.

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Antisense Technology, Part B by John N. Abelson, Melvin I. Simon, M. Ian Phillips

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